Here you can see the green shining Bruchpilot gene of a fruit fly. (STED microscopy)

Neuroscience Microscopes

These systems are preferentially accessible to projects associated with NeuroCure.

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The following systems are available:

Confocal microscope SP5

Optimized for fixed samples

 

Excitation

Epifluorescence UV (BP 340-380 nm)

Epifluorescence blue (BP 450-490 nm)

Epifluorescence green (BP 515-560 nm)

 

Detection

3 PMT 400 - 800nm spectral descanned

2 APD FITC/TRITC

Emission blue  (LP 425 nm)

Emission green (LP 515 nm)

Emission red (LP 590 nm)

 

Equipment

Upright microscope (Leica DM 6000)

Laser diode (405 nm)

Single photon Ar laser (458, 476, 488,496, 514 nm)

Solid state laser (561 nm)

HeNe laser (633 nm)

Immersion objective: 20x,

Oil immersion objective: 63x 

Mechanical X-Y table

Confocal microscope SP8

Optimized for fixed samples

 

Excitation:

Epifluorescence UV (BP 340-380 nm)

Epifluorescence blue (BP 450-490 nm)

Epifluorescence green (BP 515-560 nm)

 

Detection:

2 PMT 400-800 nm spectral descanned

2 HYD (high sensitive) 400-800 nm spectral descanned

Emission blue  (LP 425 nm)

Emission green (LP 515 nm)

Emission red (LP 590 nm)

 

 Equipment

Inverted microscope (Leica DMI 6000)

Solid state laser (405, 488, 552, 635 nm)

Immersion objective: 20x 

Oil immersion objective: 63x

Galvanic Z table, electrical X-Y table

2 Photon microscope (in vivo setup)

Optimized for in vivo samples

 

Excitation

2 photon infrared (690-1040 nm)

 

Detection

Non descanned PMT green (480-580 nm)

Non descanned PMT red (590-710 nm)

 

Equipment

Upright microscope (Olympus BX 61)

Water immersion objectives: 10x and 40x

Electrical table and electrical micromanipulators

2 Photon microscope (in vitro setup)

Optimized for in vitro samples

 

Excitation

2 Photon infrared (710-950 nm) and

2 Photon infrared (720-950 nm)

 

Detection

Non descanned PMT green (480-580 nm)

Non descanned PMT red (590-710 nm)

 

Equipment

Upright microscope (Olympus BX 61)

Water-immersion objectives: 10x und 40x

Electric table and electric micromanipulators

Gated STED system

Optimized for fixed samples

 

Excitation

Epifluorescence blue (BP 450-490 nm)

Epifluorescence green (BP 515-560nm)

 

Detection

2 PMT 400-800nm spectral descanned

2 HYD (high sensitive) 400-800nm spectral descanned

Emission blue  (LP 425 nm)

Emission green (LP 515 nm)

Emission red (LP 590 nm)

 

Equipment

inverted (Leica DMI 6000)

Single photon Ar Laser (458, 476, 488, 496, 514 nm)

Solid State Laser (568 nm),

HeNe laser (633nm)

CW gated STED Laser (592 nm)

White light Laser (450-650 nm)

Dry objektiv: 10x

Immersion objective: 20x

Oil-immersion objective: 63x

Galvanic Z- table, mechanic XY- table

Trained users can book time slots two weeks in advance:

Before starting:

The use of our Systems is only permitted after filling out an application and an initial training.

Contact:

Jörg Rösner

+49 30 450 628 203